Abstract
Objective Natural CD4+CD25+FoxP3+ Treg cells play a crucial role in maintaining immune homeostasis and controlling autoimmunity. In patients with juvenile idiopathic arthritis (JIA), inflammation occurs despite the increased total numbers of Treg cells in the synovial fluid (SF) compared to the peripheral blood (PB). This study was undertaken to investigate the phenotype of CD4+ T cells in PB and SF from JIA patients, the function of synovial Treg cells, and the sensitivity of PB and SF CD4+CD25- effector T cells to the immunoregulatory properties of Treg cells, and to study the suppression of cytokine secretion from SF effector T cells by Treg cells. Methods The phenotypes of effector T cells and Treg cells of PB and SF from JIA patients and healthy donors were determined by flow cytometry. The functionality of isolated Treg cells and effector T cells was quantified in 3H-thymidine proliferation assays. Cytokine levels were analyzed using Bio-Plex Pro assay. Results Compared to PB, SF showed significantly elevated numbers of activated and differentiated CD4+CD45RO+ T cells. Sensitivity of SF effector T cells to the suppressive effects of Treg cells from both PB and SF was impaired, correlating inversely with the expression of CD69 and HLA-DR. However, SF effector T cell cytokine secretion was partly suppressed by SF Treg cells. Conclusion Our findings indicate that regulation is impaired in the SF of patients with JIA, as shown by the resistance of effector T cells to immunoregulation by functional Treg cells. This resistance of the SF effector T cells might be due to their activated phenotype.
| Original language | English |
|---|---|
| Pages (from-to) | 3153-3162 |
| Number of pages | 10 |
| Journal | Arthritis and Rheumatism |
| Volume | 63 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2011 |
| Externally published | Yes |
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Haufe, S., Haug, M., Schepp, C., Kuemmerle-Deschner, J., Hansmann, S., Rieber, N., Tzaribachev, N., Hospach, T., Maier, J., Dannecker, G. E., & Holzer, U. (2011). Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells. Arthritis and Rheumatism, 63(10), 3153-3162. https://doi.org/10.1002/art.30503
Haufe, Susanne ; Haug, Markus ; Schepp, Carsten et al. / Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells. In: Arthritis and Rheumatism. 2011 ; Vol. 63, No. 10. pp. 3153-3162.
@article{612e29ec4d0b4d949252bd8a44c20deb,
title = "Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells",
abstract = "Objective Natural CD4+CD25+FoxP3+ Treg cells play a crucial role in maintaining immune homeostasis and controlling autoimmunity. In patients with juvenile idiopathic arthritis (JIA), inflammation occurs despite the increased total numbers of Treg cells in the synovial fluid (SF) compared to the peripheral blood (PB). This study was undertaken to investigate the phenotype of CD4+ T cells in PB and SF from JIA patients, the function of synovial Treg cells, and the sensitivity of PB and SF CD4+CD25- effector T cells to the immunoregulatory properties of Treg cells, and to study the suppression of cytokine secretion from SF effector T cells by Treg cells. Methods The phenotypes of effector T cells and Treg cells of PB and SF from JIA patients and healthy donors were determined by flow cytometry. The functionality of isolated Treg cells and effector T cells was quantified in 3H-thymidine proliferation assays. Cytokine levels were analyzed using Bio-Plex Pro assay. Results Compared to PB, SF showed significantly elevated numbers of activated and differentiated CD4+CD45RO+ T cells. Sensitivity of SF effector T cells to the suppressive effects of Treg cells from both PB and SF was impaired, correlating inversely with the expression of CD69 and HLA-DR. However, SF effector T cell cytokine secretion was partly suppressed by SF Treg cells. Conclusion Our findings indicate that regulation is impaired in the SF of patients with JIA, as shown by the resistance of effector T cells to immunoregulation by functional Treg cells. This resistance of the SF effector T cells might be due to their activated phenotype.",
author = "Susanne Haufe and Markus Haug and Carsten Schepp and Jasmin Kuemmerle-Deschner and Sandra Hansmann and Nikolaus Rieber and Nikolay Tzaribachev and Toni Hospach and Jan Maier and Dannecker, {Guenther E.} and Ursula Holzer",
year = "2011",
month = oct,
doi = "10.1002/art.30503",
language = "English",
volume = "63",
pages = "3153--3162",
journal = "Arthritis and Rheumatism",
issn = "0004-3591",
publisher = "John Wiley & Sons Inc.",
number = "10",
}
Haufe, S, Haug, M, Schepp, C, Kuemmerle-Deschner, J, Hansmann, S, Rieber, N, Tzaribachev, N, Hospach, T, Maier, J, Dannecker, GE & Holzer, U 2011, 'Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells', Arthritis and Rheumatism, vol. 63, no. 10, pp. 3153-3162. https://doi.org/10.1002/art.30503
Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells. / Haufe, Susanne; Haug, Markus; Schepp, Carsten et al.
In: Arthritis and Rheumatism, Vol. 63, No. 10, 10.2011, p. 3153-3162.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells
AU - Haufe, Susanne
AU - Haug, Markus
AU - Schepp, Carsten
AU - Kuemmerle-Deschner, Jasmin
AU - Hansmann, Sandra
AU - Rieber, Nikolaus
AU - Tzaribachev, Nikolay
AU - Hospach, Toni
AU - Maier, Jan
AU - Dannecker, Guenther E.
AU - Holzer, Ursula
PY - 2011/10
Y1 - 2011/10
N2 - Objective Natural CD4+CD25+FoxP3+ Treg cells play a crucial role in maintaining immune homeostasis and controlling autoimmunity. In patients with juvenile idiopathic arthritis (JIA), inflammation occurs despite the increased total numbers of Treg cells in the synovial fluid (SF) compared to the peripheral blood (PB). This study was undertaken to investigate the phenotype of CD4+ T cells in PB and SF from JIA patients, the function of synovial Treg cells, and the sensitivity of PB and SF CD4+CD25- effector T cells to the immunoregulatory properties of Treg cells, and to study the suppression of cytokine secretion from SF effector T cells by Treg cells. Methods The phenotypes of effector T cells and Treg cells of PB and SF from JIA patients and healthy donors were determined by flow cytometry. The functionality of isolated Treg cells and effector T cells was quantified in 3H-thymidine proliferation assays. Cytokine levels were analyzed using Bio-Plex Pro assay. Results Compared to PB, SF showed significantly elevated numbers of activated and differentiated CD4+CD45RO+ T cells. Sensitivity of SF effector T cells to the suppressive effects of Treg cells from both PB and SF was impaired, correlating inversely with the expression of CD69 and HLA-DR. However, SF effector T cell cytokine secretion was partly suppressed by SF Treg cells. Conclusion Our findings indicate that regulation is impaired in the SF of patients with JIA, as shown by the resistance of effector T cells to immunoregulation by functional Treg cells. This resistance of the SF effector T cells might be due to their activated phenotype.
AB - Objective Natural CD4+CD25+FoxP3+ Treg cells play a crucial role in maintaining immune homeostasis and controlling autoimmunity. In patients with juvenile idiopathic arthritis (JIA), inflammation occurs despite the increased total numbers of Treg cells in the synovial fluid (SF) compared to the peripheral blood (PB). This study was undertaken to investigate the phenotype of CD4+ T cells in PB and SF from JIA patients, the function of synovial Treg cells, and the sensitivity of PB and SF CD4+CD25- effector T cells to the immunoregulatory properties of Treg cells, and to study the suppression of cytokine secretion from SF effector T cells by Treg cells. Methods The phenotypes of effector T cells and Treg cells of PB and SF from JIA patients and healthy donors were determined by flow cytometry. The functionality of isolated Treg cells and effector T cells was quantified in 3H-thymidine proliferation assays. Cytokine levels were analyzed using Bio-Plex Pro assay. Results Compared to PB, SF showed significantly elevated numbers of activated and differentiated CD4+CD45RO+ T cells. Sensitivity of SF effector T cells to the suppressive effects of Treg cells from both PB and SF was impaired, correlating inversely with the expression of CD69 and HLA-DR. However, SF effector T cell cytokine secretion was partly suppressed by SF Treg cells. Conclusion Our findings indicate that regulation is impaired in the SF of patients with JIA, as shown by the resistance of effector T cells to immunoregulation by functional Treg cells. This resistance of the SF effector T cells might be due to their activated phenotype.
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U2 - 10.1002/art.30503
DO - 10.1002/art.30503
M3 - Article
C2 - 21702013
AN - SCOPUS:80053493638
SN - 0004-3591
VL - 63
SP - 3153
EP - 3162
JO - Arthritis and Rheumatism
JF - Arthritis and Rheumatism
IS - 10
ER -
Haufe S, Haug M, Schepp C, Kuemmerle-Deschner J, Hansmann S, Rieber N et al. Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells. Arthritis and Rheumatism. 2011 Oct;63(10):3153-3162. doi: 10.1002/art.30503
